pCW57.1 Lysosomal-METRIQ | Addgene 135401 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCW57.1 Lysosomal-METRIQ

catalog :

135401

citations: 2

Reference
Carb xf3 J, Cornet Masana J, Cuesta Casanovas L, Delgado Mart xed nez J, Ban xfa s Mulet A, Cl xe9 ment Demange L, et al. A Novel Family of Lysosomotropic Tetracyclic Compounds for Treating Leukemia. Cancers (Basel). 2023;15: pubmed publisher
Ishii S, Matsuura A, Itakura E. Identification of a factor controlling lysosomal homeostasis using a novel lysosomal trafficking probe. Sci Rep. 2019;9:11635 pubmed publisher

product information

Catalog Number :

135401

Product Name :

pCW57.1 Lysosomal-METRIQ

article :

doi	10.1038/s41598-019-48131-2
id	28207191
pubmed_id	31406169

bacterial resistance :

Ampicillin

cloning :

backbone

pMRX-IP

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth strain :

Expresses lysosomal-METRIQ (DNAse II-sfGFP together with cytosolic RFP) to measure lysosomal activity

origin :

pi :

DNL, DNL2geneDNASE2id1777genbank_ids

NM_001375.2

mutationSingle-nucleotide polymorphism C432TnameDNAse II alphashRNA_sequencesize1080species

9606

Homo sapiens

tags

location	C terminal on insert
tag	sfGFP

location	C terminal on insert
tag	T2A

location
tag	mCherry

Cloningcloning_site_3
cloning_site
_5promotertd>sequencing_pri
mer_3sequenc
ing_primer_5GATCGCCTGGAGAATTGG
accATGggcatcccgctgctgctggcag<
tr>site_3_destroyedtr>site_5_destroyedd>entrez
_gene

aliases

DNASE2A,

alt_names

DNASE2

Deoxyribonuclease-2-alpha

cloning

clone_method

Gibson

resistance markers :

4728

tags :

Unknown

terms :

Puromycin