This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
3xIRS luciferase
catalog :
13511
citations: 2
Reference
Pyrzynska B, Dwojak M, Zerrouqi A, Morlino G, Zapala P, Miazek N, et al. FOXO1 promotes resistance of non-Hodgkin lymphomas to anti-CD20-based therapy. Oncoimmunology. 2018;7:e1423183 pubmed publisher
Tang E, Nunez G, Barr F, Guan K. Negative regulation of the forkhead transcription factor FKHR by Akt. J Biol Chem. 1999;274:16741-6 pubmed
product information
Catalog Number :
13511
Product Name :
3xIRS luciferase
article :
doi10.1074/jbc.274.24.16741
id1087
pubmed_id10358014
bacterial resistance :
Ampicillin
cloning :
backbonepGL2-Promoter
backbone_mutation
backbone_originPromega
backbone_size5800
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Luciferase
growth notes :
and TCGATTCAAAATAAGTTTGTTTTGCTTCAAAATAAGTTTGTTTTGCTTCAAAATAAGTTTGTTTTGCGTAC) were annealed together and ligated into the pGL2-Promoter vector (Promega) to create 3 IRS-luciferase. The IRS sequences were derived from human IGFBP-1's IRS. See Author's Map for more information.
(GCAAAACAAACTTATTTTGAAGCAAAACAAACTTATTT
TGAAGCAAAACAAACTTATTTTGAA
Two oligonucleotides
origin :
37
pi :
alt_names
Insulin-responsive sequence
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5KpnI
promoter
sequencing_primer_3LucNrev primer
sequencing_primer_5EBV rev primer
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesAFBP, IBP1, IGF-BP25, PP12, hIGFBP-1
geneIGFBP1
id3484
genbank_ids
mutation
name3xIRS
shRNA_sequence
size150
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagluciferase
resistance markers :
719
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA