Myo 1b-myc | Addgene 135064 product information

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product summary

company name :

Addgene

product type :

cDNA

product name :

Myo 1b-myc

catalog :

135064

product information

Catalog Number :

135064

Product Name :

Myo 1b-myc

article :

doi	10.1083/jcb.120.6.1393
id	28207134
pubmed_id	8449985

bacterial resistance :

Ampicillin

cloning :

backbone

pCMV5

backbone_mutation

backbone_origin

Dr. David Russell, UTSW

backbone_size

4603

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Myr 1 has three alternative spliceforms. In this plasmid spliceform Myr 1b is cloned, that exhibits a deletion of 29 amino acids. The size of the 5' UTR is unknown, therefore the Insert size is estimated. See also : Ruppert C, Godel J, Muller RT, Kroschewski R, Reinhard J, Bahler M J Cell Sci. 1995 Dec;108 ( Pt 12):3775-86. PubMed

growth strain :

Expression of rat Myo 1b (Myr 1) in mammalian cells. C-terminal myc-tag.

origin :

pi :

alt_names

Myr 1

cloning

clone_method	Restriction Enzyme
cloning_site_3	Xba I
cloning_site_5	Eco RI
promoter	CMV
sequencing_primer_3	hGH-PA-R CCAGCTTGGTTCCCAATAGA
sequencing_primer_5	CMV-F CGCAAATGGGCGGTAGGCGTG
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Myr1
gene	Myo1b
id	117057

genbank_ids

X68199

mutation

name

Myo 1b with 5' UTR

shRNA_sequence

size

3600

species

10116

Rattus norvegicus