This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGEX4T1-Smurf1 C699A
catalog :
13503
citations: 1
Reference
Zhu H, Kavsak P, Abdollah S, Wrana J, Thomsen G. A SMAD ubiquitin ligase targets the BMP pathway and affects embryonic pattern formation. Nature. 1999;400:687-93 pubmed
product information
Catalog Number :
13503
Product Name :
pGEX4T1-Smurf1 C699A
article :
doi10.1038/23293
id654
pubmed_id10458166
bacterial resistance :
Ampicillin
cloning :
backbonepGEX-4T1
backbone_mutation
backbone_origin
backbone_size4900
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Vector has TEV site. A human Smurf1 cDNA encoding all but the first eight amino acids was identified in the expressed-sequence-tag database (AA292123), and full-length hSmurf1 was reconstituted using a fragment of XSmurf1 cDNA encoding the first eight amino acids.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5SalI
promoter
sequencing_primer_3
sequencing_primer_5pGEX 5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneSMURF1
id57154
genbank_ids
NM_020429
mutationC699A: catalytically inactive
nameSMURF1
shRNA_sequence
size2100
species
9606
Homo sapiens
tags
locationN terminal on insert
tagGST
resistance markers :
589
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA