This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLKO5-SgHottip-GFP-CRISPRa
catalog :
134990
citations: 1
Reference
Luo H, Zhu G, Xu J, Lai Q, Yan B, Guo Y, et al. HOTTIP lncRNA Promotes Hematopoietic Stem Cell Self-Renewal Leading to AML-like Disease in Mice. Cancer Cell. 2019;36:645-659.e8 pubmed publisher
product information
Catalog Number :
134990
Product Name :
pLKO5-SgHottip-GFP-CRISPRa
article :
doi10.1016/j.ccell.2019.10.011
id28207119
pubmed_id31786140
bacterial resistance :
Ampicillin
cloning :
backbonepLKO5.sgRNA.EFS.GFP
backbone_mutationBsmBI cut for SgRNA insert
backbone_originBenjamin Ebert LAB
backbone_size7408
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Bacterial Expression
Lentiviral
growth strain :
dCas9-mediated activation of HOTTIP in mammalian cells
origin :
37
pi :
alt_names
HOXA-AS6
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterU6
sequencing_primer_3
sequencing_primer_5GACTATCATATGCTTACCGT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHOXA-AS6, HOXA13-AS1, NCRNA00213
geneHOTTIP
id100316868
genbank_ids
NC_000007.14
mutation
nameHOTTIP
shRNA_sequenceGGCCGCGCACCATTCACCCG
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGFP
resistance markers :
4708
tags :
High Copy
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA