Myo 9 Avi+Flag | Addgene 134969 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Myo 9 Avi+Flag

catalog :

134969

citations: 1

Reference
Liao W, Elfrink K, Bähler M. Head of myosin IX binds calmodulin and moves processively toward the plus-end of actin filaments. J Biol Chem. 2010;285:24933-42 pubmed publisher

product information

Catalog Number :

134969

Product Name :

Myo 9 Avi+Flag

article :

doi	10.1074/jbc.M110.101105
id	28207114
pubmed_id	20538589

bacterial resistance :

Ampicillin

cloning :

backbone

pFast Bac 1

backbone_mutation

Avi and Flag tag

backbone_origin

Invitrogen

backbone_size

4673

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Insect Expression

growth notes :

Sequencing of the C. elegans Myo9 cDNA that was obtained by reverse transcription-PCR revealed that it differed from the HUM-7 amino acid sequence at two positions. Residues 608 615 (VSPISPFW) were missing, and residues 731KKSAG735 were replaced by 731KSESAG736. Our deduced Myo9 amino acid sequence changes matched perfectly with the predicted Myo9 sequences for Caenorhabditis briggsae and Caenorhabditis remanei.

growth strain :

Expression and purification of c.elegans Myo 9 (Hum 7) using baculovirus from pFastBac1

origin :

pi :

alt_names

Hum 7, Myo IX

cloning

clone_method	Restriction Enzyme
cloning_site_3	Kpn I
cloning_site_5	Bam HI
promoter	Polyhedrin
sequencing_primer_3	SV40pA-R GAAATTTGTGATGCTATTGC
sequencing_primer_5	Polyhedrin forward AAATGATAACCATCTCGC
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CELE_F56A6.2
gene	hum-7
id	171650

genbank_ids

mutation

name

Myo 9

shRNA_sequence

size

5740

species

6239

Caenorhabditis elegans