This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
mcherry-Myo 9b R1695M
catalog :
134958
citations: 1
Reference
Hemkemeyer S, Vollmer V, Schwarz V, Lohmann B, Honnert U, Taha M, et al. Local Myo9b RhoGAP activity regulates cell motility. J Biol Chem. 2021;296:100136 pubmed publisher
product information
Catalog Number :
134958
Product Name :
mcherry-Myo 9b R1695M
article :
doi10.1074/jbc.RA120.013623
id28207099
pubmed_id33268376
bacterial resistance :
Kanamycin
cloning :
backbonepmcherry-C1 (modified)
backbone_mutationpmcherry-C1 (Clontech), NT 597-612 and NT 1321-1329 are missing (no Eco 47 III, no Age I, no BspE I site).
backbone_originClontech (modified)
backbone_size4652
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please note: plasmid contains a T721I mutation in Myo9b. This mutation is not known to affect plasmid function.
growth strain :
Expression of rat Myo 9b (Myr 5) in mammalian cells.GAP minus mutant.
origin :
37
pi :
alt_names
Myr 5 R1695M
cloning
clone_methodRestriction Enzyme
cloning_site_3Sma I
cloning_site_5Xho I
promoterCMV IE
sequencing_primer_3SV40pA-R GAAATTTGTGATGCTATTGC
sequencing_primer_5mcherry-F CCCCGTAATGCAGAAGAAGA
site_3_destroyed1
site_5_destroyed
entrez_gene
aliases
geneMyo9b
id25486
genbank_ids
mutation
nameMyo 9b (rat) with 3' UTR. GAP minus mutant R1695M
shRNA_sequence
size6849
species
10116
Rattus norvegicus
tags
locationN terminal on backbone
tagmcherry
resistance markers :
4726
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA