This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBABE MDproER
catalog :
13495
citations: 1
Reference
Rosenberg M, Georges S, Asawachaicharn A, Analau E, Tapscott S. MyoD inhibits Fstl1 and Utrn expression by inducing transcription of miR-206. J Cell Biol. 2006;175:77-85 pubmed
product information
Catalog Number :
13495
Product Name :
pBABE MDproER
article :
doi10.1083/jcb.200603039
id1085
pubmed_id17030984
bacterial resistance :
Ampicillin
cloning :
backbonepBABE puro
backbone_mutation
backbone_originMorgenstern&Land (NAR 18:3587); Available at Addgene
backbone_size5100
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
The Entrez ID for MyoD is NM_010866 ; the Entrez ID for ER is NM_000125. See pBABE MDER for map of wild-type plasmid.
origin :
37
pi :
alt_names
Mouse Myod1
ESR1
Myod1
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5EcoRI
promoter
sequencing_primer_3
sequencing_primer_5pBABE 5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMYF3, MyoD, Myod-1, bHLHc1
geneMyod1
id17927
aliasesER, ESR, ESRA, ESTRR, Era, NR3A1
geneESR1
id2099
genbank_ids
NM_010866
mutationfull length MyoD (containing the point mutation A114P) was fused to aa282-595 of Esr1. Esr1 is inserted between codons 173 and 174 of MyoD.
nameMyoD1-Estrogen Receptor Fusion Protein
shRNA_sequence
size2700
species
9606
Homo sapiens
10090
Mus musculus
tags
resistance markers :
520
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA