This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
GFP-Myo9b R1695M
catalog :
134911
product information
Catalog Number :
134911
Product Name :
GFP-Myo9b R1695M
article :
doi10.1091/mbc.8.10.2039
id28207096
pubmed_id9348541
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1
backbone_mutation
backbone_originClontech
backbone_size4686
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
See also: Van den Boom et al. Molecular Biology of the Cell Vol. 18, No. 4, 1507-1518, April 2007 The Myosin IXb Motor Activity Targets the Myosin IXb RhoGAP Domain as Cargo to Sites of Actin Polymerization. Published Online:21 Feb 2007https://doi.org/10.1091/mbc.e06-08-0771 Please note: Plasmid contains a T721I mutation in Myo9b. This mutation is not known to affect protein function.
growth strain :
Expression of rat Myo 9b (Myr 5) in mammalian cells.GAP minus mutant.
origin :
37
pi :
alt_names
Myr 5 R1695M
cloning
clone_methodRestriction Enzyme
cloning_site_3Sma I
cloning_site_5Xho I
promoterCMV IE
sequencing_primer_3SV40pA-R GAAATTTGTGATGCTATTGC
sequencing_primer_5EGFP-C CATGGTCCTGCTGGAGTTCGTG
site_3_destroyed1
site_5_destroyed
entrez_gene
aliases
geneMyo9b
id25486
genbank_ids
mutationchanged arginine 1695 to methionine
nameMyo 9b (rat) with 3' UTR. GAP minus mutant R1695M
shRNA_sequence
size6849
species
10116
Rattus norvegicus
tags
locationN terminal on backbone
tagEGFP
resistance markers :
4726
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA