This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET16b-PCNA
catalog :
134898
citations: 1
Reference
Tan W, Murphy V, Charron A, van Twest S, Sharp M, Constantinou A, et al. Preparation and purification of mono-ubiquitinated proteins using Avi-tagged ubiquitin. PLoS ONE. 2020;15:e0229000 pubmed publisher
product information
Catalog Number :
134898
Product Name :
pET16b-PCNA
article :
doi10.1371/journal.pone.0229000
id28207089
pubmed_id32092106
bacterial resistance :
Ampicillin
cloning :
backbonepET16b
backbone_mutation
backbone_originNovagen
backbone_size5711
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
This is a codon optimized untagged PCNA cDNA expression vector. N- or C-terminal tags on PCNA inhibit its trimerization, so purification is instead via several steps of anion exchange and size exclusion chromatography. Protein purification protocol is as in Tan et al. Plos One 2020
growth strain :
Bacterial expression and purification of human PCNA
origin :
37
pi :
alt_names
Proliferating Cell Nuclear Antigen
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterT7
sequencing_primer_3T7 terminator
sequencing_primer_5T7 promoter
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesATLD2
genePCNA
id5111
genbank_ids
mutationfully synthetic, codon optimized for bacterial expression
namePCNA
shRNA_sequence
size783
species
9606
Homo sapiens
tags
location
taguntagged
resistance markers :
4857
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA