This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
mSin1 shRNA #2
catalog :
13484
citations: 2
Reference
Carbonneau M, M Gagné L, Lalonde M, Germain M, Motorina A, Guiot M, et al. The oncometabolite 2-hydroxyglutarate activates the mTOR signalling pathway. Nat Commun. 2016;7:12700 pubmed publisher
Frias M, Thoreen C, Jaffe J, Schroder W, Sculley T, Carr S, et al. mSin1 is necessary for Akt/PKB phosphorylation, and its isoforms define three distinct mTORC2s. Curr Biol. 2006;16:1865-70 pubmed
product information
Catalog Number :
13484
Product Name :
mSin1 shRNA #2
article :
doi10.1016/j.cub.2006.08.001
id925
pubmed_id16919458
bacterial resistance :
Ampicillin
cloning :
backbonepLKO.1
backbone_mutation
backbone_origin
backbone_size7032
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
RNAi
growth notes :
Short hairpin construct specific for human mSin1 (mSin1.1#2, TRC shRNA NM_024117.x-1308s1c1) from the RNAi Consortium. The target sequence is 5'-CCACA GTACA GGATA TGCTT-3'.
origin :
37
pi :
alt_names
shRNA mSin1
MAPKAP1
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5AgeI
promoter
sequencing_primer_3
sequencing_primer_5LKO.1 5'
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesJC310, MIP1, SIN1, SIN1b, SIN1g
geneMAPKAP1
id79109
genbank_ids
NM_024117
mutation
namemSin1
shRNA_sequence
size60
species
9606
Homo sapiens
tags
resistance markers :
432
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA