This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLenti CMV Puro DHFR.dn-cHSF1
catalog :
134729
citations: 1
Reference
Moore C, Dewal M, Nekongo E, Santiago S, Lu N, Levine S, et al. Transportable, Chemical Genetic Methodology for the Small Molecule-Mediated Inhibition of Heat Shock Factor 1. ACS Chem Biol. 2016;11:200-10 pubmed publisher
product information
Catalog Number :
134729
Product Name :
pLenti CMV Puro DHFR.dn-cHSF1
article :
doi10.1021/acschembio.5b00740
id28207033
pubmed_id26502114
bacterial resistance :
Ampicillin
cloning :
backbonepLenti CMV Puro Dest (w118-1)
backbone_mutation
backbone_originEric Campeau, Paul Kaufman
backbone_size8022
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
Lentiviral expression vector for constitutively active dominant-negative HSF1 fused to DHFR destabilized domain
origin :
30
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3CATAGCGTAAAAGGAGCAACA
sequencing_primer_5CGCAAATGGGCGGTAGGCGTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHSTF1
geneHSF1
id3297
genbank_ids
NM_005526.4
mutationDeletion of amino acids 186-202, 379 529
nameDHFR.dn-cHSF1
shRNA_sequence
size1593
species
9606
Homo sapiens
tags
locationN terminal on insert
tagDHFR
resistance markers :
4624
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA