This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.1.G s-LgB113
catalog :
134362
citations: 1
Reference
Laschet C, Dupuis N, Hanson J. A dynamic and screening-compatible nanoluciferase-based complementation assay enables profiling of individual GPCR-G protein interactions. J Biol Chem. 2019;294:4079-4090 pubmed publisher
product information
Catalog Number :
134362
Product Name :
pcDNA3.1.G s-LgB113
article :
doi10.1074/jbc.RA118.006231
id28207007
pubmed_id30593506
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1
backbone_mutation
backbone_origin
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Bacterial Expression
growth strain :
Nanoluc complementation assay. Expression of G s protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 113 and 114 of G s. Addition of the HA epitope at N terminus of G s.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5HindIII
promoterT7
sequencing_primer_3TTCCCAATCCTCCCCCTTG
sequencing_primer_5TAATACGACTCACTATAGGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAHO, C20orf45, GNAS1, GPSA, GSA, GSP, NESP, PITA3, POH, SCG6, SgVI
geneGNAS
id2778
genbank_ids
mutation
nameG s-LgB113
shRNA_sequence
size1734
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHA
resistance markers :
4725
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA