This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.1.G q-LgB97
catalog :
134360
citations: 1
Reference
Laschet C, Dupuis N, Hanson J. A dynamic and screening-compatible nanoluciferase-based complementation assay enables profiling of individual GPCR-G protein interactions. J Biol Chem. 2019;294:4079-4090 pubmed publisher
product information
Catalog Number :
134360
Product Name :
pcDNA3.1.G q-LgB97
article :
doi10.1074/jbc.RA118.006231
id28207007
pubmed_id30593506
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1
backbone_mutation
backbone_origin
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Bacterial Expression
growth strain :
Nanoluc complementation assay. Expression of G q protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of G q. Addition of the HA epitope at N terminus of G q.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5HindIII
promoterT7
sequencing_primer_3TTCCCAATCCTCCCCCTTG
sequencing_primer_5TAATACGACTCACTATAGGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCMAL, CMC1, G-ALPHA-q, GAQ, SWS
geneGNAQ
id2776
genbank_ids
NM_002072.5
mutation
nameG q-LgB97
shRNA_sequence
size1629
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHA
resistance markers :
4725
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA