This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
AAVS1-TRE-SHH
catalog :
134350
citations: 1
Reference
Cederquist G, Asciolla J, Tchieu J, Walsh R, Cornacchia D, Resh M, et al. Specification of positional identity in forebrain organoids. Nat Biotechnol. 2019;37:436-444 pubmed publisher
product information
Catalog Number :
134350
Product Name :
AAVS1-TRE-SHH
article :
doi10.1038/s41587-019-0085-3
id28206788
pubmed_id30936566
bacterial resistance :
Ampicillin
cloning :
backbonePuro-iDest
backbone_mutation
backbone_origin
backbone_size8904
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Puro-iDEST (Addgene #75336)
growth strain :
Targeting vector for site specific integration of doxycycline inducible expression of full length human SHH cDNA into human AAVS1 locus
origin :
30
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3GAAATTTGTGATGCTATTGC (SV40pA-R)
sequencing_primer_5GTTTGTACAAAAAAGCAGGC (ATTB1)
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHHG1, HLP3, HPE3, MCOPCB5, SMMCI, ShhNC, TPT, TPTPS
geneSHH
id6469
genbank_ids
NM_000193.3
mutation
nameSHH
shRNA_sequence
size1389
species
9606
Homo sapiens
tags
plasmid copy :
human SHH cDNA was purchased from Genecopoeia, Product ID:T1004
resistance markers :
3770
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA