This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLV-puro-N-3HA-SREBP2-C-Flag
catalog :
134287
citations: 1
Reference
Zhang D, Tomisato W, Su L, Sun L, Choi J, Zhang Z, et al. Skin-specific regulation of SREBP processing and lipid biosynthesis by glycerol kinase 5. Proc Natl Acad Sci U S A. 2017;114:E5197-E5206 pubmed publisher
product information
Catalog Number :
134287
Product Name :
pLV-puro-N-3HA-SREBP2-C-Flag
article :
doi10.1073/pnas.1705312114
id28194198
pubmed_id28607088
bacterial resistance :
Ampicillin
cloning :
backbonepLV-EF1a -MCS-IRES-Puro
backbone_mutation
backbone_originBioSettia
backbone_size8900
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
Lentivector encoding 3XHA (N-term) and Flag (C-term)-tagged SREBP2
origin :
37
pi :
alt_names
Srebf2; bHLHd2; SREBP-2
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3ttaccctgttatccctaggc
sequencing_primer_5cttccatttcaggtgtcgtg
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesSREBP-2, SREBP2, SREBP2gc, bHLHd2, lop13, nuc
geneSrebf2
id20788
genbank_ids
NM_033218
mutationmouse SREBP2 precursor
nameSREBP2
shRNA_sequence
size3393
species
10090
Mus musculus
tags
locationC terminal on insert
tagFlag
locationN terminal on insert
tag3x HA
resistance markers :
33
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA