This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pFSW Syt1-C2A D230,232N IRES GFP
catalog :
133824
citations: 1
Reference
Courtney N, Bao H, Briguglio J, Chapman E. Synaptotagmin 1 clamps synaptic vesicle fusion in mammalian neurons independent of complexin. Nat Commun. 2019;10:4076 pubmed publisher
product information
Catalog Number :
133824
Product Name :
pFSW Syt1-C2A D230,232N IRES GFP
article :
doi10.1038/s41467-019-12015-w
id28206954
pubmed_id31501440
bacterial resistance :
Ampicillin
cloning :
backboneFUGW
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Lentiviral
growth notes :
5' Cloning Site: NheI (destroyed during cloning), 3' Cloning Site: EcoRI (destroyed during cloning)
growth strain :
Encodes the C2A domain of synaptotagmin 1 with calcium-coordinating ligand mutations D230,232N for viral expression
origin :
37
pi :
alt_names
Synaptotagmin 1
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterhSyn (human synapsin I promoter)
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesP65
geneSyt1
id25716
genbank_ids
mutationD230,232N
nameSyt1
shRNA_sequence
size789
species
10116
Rattus norvegicus
tags
locationC terminal on backbone
tagIRES-GFP
resistance markers :
4536
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA