This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAAV-EF1a-DIO-PSD95-EGFP-WPRE
catalog :
133785
citations: 2
Reference
Gobbo F, Cattaneo A. Neuronal Activity at Synapse Resolution: Reporters and Effectors for Synaptic Neuroscience. Front Mol Neurosci. 2020;13:572312 pubmed publisher
Sun Y, Espinosa J, Hoseini M, Stryker M. Experience-dependent structural plasticity at pre- and postsynaptic sites of layer 2/3 cells in developing visual cortex. Proc Natl Acad Sci U S A. 2019;116:21812-21820 pubmed publisher
product information
Catalog Number :
133785
Product Name :
pAAV-EF1a-DIO-PSD95-EGFP-WPRE
article :
doi10.1073/pnas.1914661116
id28206948
pubmed_id31591211
bacterial resistance :
Ampicillin
cloning :
backbonepAAV-EF1a-double floxed-TdTomato-WPRE
backbone_mutation
backbone_originkarl deisseroth
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
AAV
Cre/Lox
growth notes :
Please note: Plasmid contains FS793-794LT, A892V, and H932T mutations in the insert sequence, which all fall within EGFP. These mutations are not known to affect plasmid function.
growth strain :
Cre-dependent EGFP Fluorescent reporter for overexpressed postsynaptic marker PSD95 in mammalian cell
origin :
37
pi :
alt_names
cloning
entrez_gene
aliasesDlgh4, PSD95, Sap90
geneDlg4
id29495
genbank_ids
NM_019621.1
mutation
namePSD95-EGFP
shRNA_sequence
size2241
species
10116
Rattus norvegicus
tags
locationC terminal on insert
tagEGFP
resistance markers :
2746
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA