This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFP-C1-RhebL1
catalog :
133766
citations: 1
Reference
Angarola B, Ferguson S. Weak membrane interactions allow Rheb to activate mTORC1 signaling without major lysosome enrichment. Mol Biol Cell. 2019;30:2750-2760 pubmed publisher
product information
Catalog Number :
133766
Product Name :
pEGFP-C1-RhebL1
article :
doi10.1091/mbc.E19-03-0146
id28206938
pubmed_id31532697
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1
backbone_mutation
backbone_originClontech
backbone_size4500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please visit https://www.biorxiv.org/content/10.1101/513473v3 for bioRxiv preprint.
growth strain :
Expresses GFP-tagged RhebL1 in mammalian cells
origin :
37
pi :
alt_names
Ras Homolog Enriched In Brain-Like Protein 1
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3GAAATTTGTGATGCTATTGC
sequencing_primer_5CATGGTCCTGCTGGAGTTCGTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesRHEBL1c
geneRHEBL1
id121268
genbank_ids
NM_144593.3
mutation
nameRhebL1
shRNA_sequence
size552
species
9606
Homo sapiens
tags
locationN terminal on insert
tagEGFP
resistance markers :
1299
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA