PWPXLD-EGFR-P667A | Addgene 133750 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

PWPXLD-EGFR-P667A

catalog :

133750

citations: 1

Reference
Abdi K, Neves G, Pyun J, Kiziltug E, Ahrens A, Kuo C. EGFR Signaling Termination via Numb Trafficking in Ependymal Progenitors Controls Postnatal Neurogenic Niche Differentiation. Cell Rep. 2019;28:2012-2022.e4 pubmed publisher

product information

Catalog Number :

133750

Product Name :

PWPXLD-EGFR-P667A

article :

doi	10.1016/j.celrep.2019.07.056
id	28206936
pubmed_id	31433979

bacterial resistance :

Ampicillin

cloning :

backbone

PWPXLD

backbone_mutation

backbone_origin

backbone_size

10455

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

The mutation in this human EGFR cDNA corresponds to the conversion of proline 667 to alanine described in Cheng He et al., JBC, 2002. In this larger isoform of human EGFR the proline is located at 691. The site of the mutation is 685-RELVEP-691.

growth strain :

EGFR with mutation at proline 667 converted to alanine and tagged with HA cloned into PWPXLD plasmid

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	SPE1
cloning_site_5	MLU1
promoter	EF1-alpha
sequencing_primer_3	GCGTAAAAGGAGCAACATAG
sequencing_primer_5	CAAGCCTCAGACAGTGGTTC
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ERBB, ERBB1, ERRP, HER1, NISBD2, PIG61, mENA
gene	EGFR
id	1956

genbank_ids

NM_005228

mutation

proline 667 converted to alanine (Please see depositor comments below)

name

Mutant P667A EGFR-HA

shRNA_sequence

size

3633

species

9606

Homo sapiens