TfR-sfGFP-myc tag-SpyCatcher003 | Addgene 133451 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

TfR-sfGFP-myc tag-SpyCatcher003

catalog :

133451

citations: 3

Reference
Yang S, Klughammer N, Barth A, Tanenbaum M, Dekker C. Zero-Mode Waveguide Nanowells for Single-Molecule Detection in Living Cells. ACS Nano. 2023;17:20179-20193 pubmed publisher
Moghimianavval H, Patel C, Mohapatra S, Hwang S, Kayikcioglu T, Bashirzadeh Y, et al. Engineering Functional Membrane-Membrane Interfaces by InterSpy. Small. 2022;:e2202104 pubmed publisher
Keeble A, Turkki P, Stokes S, Khairil Anuar I, Rahikainen R, Hytönen V, et al. Approaching infinite affinity through engineering of peptide-protein interaction. Proc Natl Acad Sci U S A. 2019;: pubmed publisher

product information

Catalog Number :

133451

Product Name :

TfR-sfGFP-myc tag-SpyCatcher003

article :

doi	10.1073/pnas.1909653116
id	28206894
pubmed_id	31822621

bacterial resistance :

Kanamycin

cloning :

backbone

pENTR4

backbone_mutation

backbone_origin

backbone_size

4766

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection

origin :

pi :

alt_names

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter	CMV
sequencing_primer_3	BGH reverse
sequencing_primer_5	BGHFor (GACAGACTAACAGACTGTTCCTTTCC)
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

Contains C20 and A23 mutations that improve plasma membrane display of the transferrin receptor (TfR)

name

TfR-sfGFP-myc tag-SpyCatcher003

shRNA_sequence

size

1410

species

100

Synthetic