pst39-NST-clone14 | Addgene 133421 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pst39-NST-clone14

catalog :

133421

product information

Catalog Number :

133421

Product Name :

pst39-NST-clone14

article :

doi
id	28206891
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

pST39

backbone_mutation

backbone_origin

backbone_size

2800

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

HMS clone ID number: ScCD00061648.

growth strain :

S. cerevisiae Nip1 (with C-terminal TEV cleavage site and strep-II tag), Sui1 and Tif5B6 (with N-terminal 7xHis tag and TEV cleavage set) coding sequences with intervening ribosome binding sites and other cis-elements for expression as a polycistron, T7 theta 10 promoter (and corresponding terminator after polycistron).

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5	Unknown
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	YMR309C
gene	NIP1
id	855357

aliases	YPR041W, SUI5
gene	TIF5
id	856154

aliases	YNL244C, MOF2, RFR1
gene	SUI1
id	855477

genbank_ids

mutation

ORF for Nip1 encodes aa 4-156 (not complete ORF), ORF for Tif5 (B6) encodes aa 241-405 (not complete ORF)

name

NIP1/SUI1/TIF5

shRNA_sequence

size

species

4932

Saccharomyces cerevisiae