This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAAV2-GCaMP6f-P2A-H2B-PAmCherry
catalog :
133419
citations: 1
Reference
Lee D, Kume M, Holy T. Sensory coding mechanisms revealed by optical tagging of physiologically defined neuronal types. Science. 2019;366:1384-1389 pubmed publisher
product information
Catalog Number :
133419
Product Name :
pAAV2-GCaMP6f-P2A-H2B-PAmCherry
article :
doi10.1126/science.aax8055
id28206889
pubmed_id31831669
bacterial resistance :
Ampicillin
cloning :
backbonepAAV2
backbone_mutation
backbone_origin
backbone_size4625
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
AAV
growth notes :
pAAV2 : The Hope Center Viral Vectors Core at Washington University in St. Louis GCaMP6f :pGP-CMV-GCaMP6f was a gift from Douglas Kim & GENIE Project (Addgene plasmid # 40755 ; http://n2t.net/addgene:40755 ; RRID:Addgene_40755) PAmCherry: pPAmCherry1-C1 was a gift from Vladislav Verkhusha (Addgene plasmid # 31929 ; http://n2t.net/addgene:31929 ; RRID:Addgene_31929) H2B: H2B-GFP was a gift from Geoff Wahl (Addgene plasmid # 11680 ; http://n2t.net/addgene:11680 ; RRID:Addgene_11680)
growth strain :
Fluorescent reporter for calcium imaging and photoactivation
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterCAG
sequencing_primer_3EBV Reverse
sequencing_primer_5pCAG-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesH2B/r, H2BFR, H2BJ, HIST1H2BJ
geneH2BC11
id8970
genbank_ids
NM_021058.3
mutation
nameGCaMP6f-P2A-H2B-PAmCherry
shRNA_sequence
size2541
species
9606
Homo sapiens
32644
Other
tags
resistance markers :
4666
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA