3341 pcDNA3 Bcl-2 T69A S70A S87A | Addgene 13340 product information

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product summary

company name :

Addgene

product type :

cDNA

product name :

3341 pcDNA3 Bcl-2 T69A S70A S87A

catalog :

13340

citations: 2

Reference
Eichhorn J, Sakurikar N, Alford S, Chu R, Chambers T. Critical role of anti-apoptotic Bcl-2 protein phosphorylation in mitotic death. Cell Death Dis. 2013;4:e834 pubmed publisher
Yamamoto K, Ichijo H, Korsmeyer S. BCL-2 is phosphorylated and inactivated by an ASK1/Jun N-terminal protein kinase pathway normally activated at G(2)/M. Mol Cell Biol. 1999;19:8469-78 pubmed

product information

Catalog Number :

13340

Product Name :

3341 pcDNA3 Bcl-2 T69A S70A S87A

article :

doi
id	284
pubmed_id	10567572

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5400

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

ORF contains SacI, PstI, and BamHI sites sense from T7 direction.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	EcoRI
promoter
sequencing_primer_3
sequencing_primer_5	T7
site_3_destroyed	1
site_5_destroyed	1

entrez_gene

aliases	Bcl-2, PPP1R50
gene	BCL2
id	596

genbank_ids

NM_000633

mutation

changed Threonine 69 to Alanine; changed Serine 70 to Alanine; changed Serine 87 to Alanine

name

Bcl-2

shRNA_sequence

size

720

species

9606

Homo sapiens