This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Raf-1 GST RBD 1-149
catalog :
13338
citations: 17
Reference
Yu D, Zhao J, Lee E, Kim D, Mahapatra R, Rose E, et al. GLUT3 promotes macrophage signaling and function via RAS-mediated endocytosis in atopic dermatitis and wound healing. J Clin Invest. 2023;133: pubmed publisher
Chen L, Li Y, Chen Y. KLHL7 promotes hepatocellular carcinoma progression and molecular therapy resistance by degrading RASA2. iScience. 2023;26:106914 pubmed publisher
Kim J, Jeon Y, Lim S, Ryu J, Lee J, Chang I, et al. Akt-mediated Ephexin1-Ras interaction promotes oncogenic Ras signaling and colorectal and lung cancer cell proliferation. Cell Death Dis. 2021;12:1013 pubmed publisher
Zhou Z, Ambrogio C, Bera A, Li Q, Li X, Li L, et al. KRASQ61H preferentially signals through MAPK in a RAF dimer-dependent manner in non-small cell lung cancer. Cancer Res. 2020;: pubmed publisher
Johnson C, Lin Y, Reid D, Parker J, Pavlopoulos S, Dischinger P, et al. Isoform-Specific Destabilization of the Active Site Reveals a Molecular Mechanism of Intrinsic Activation of KRas G13D. Cell Rep. 2019;28:1538-1550.e7 pubmed publisher
Luhtala N, Hunter T. Failure to detect functional transfer of active K-Ras protein from extracellular vesicles into recipient cells in culture. PLoS ONE. 2018;13:e0203290 pubmed publisher
Koraïchi F, Gence R, Bouchenot C, Grosjean S, Lajoie Mazenc I, Favre G, et al. High-content tripartite split-GFP cell-based assays to screen for modulators of small GTPase activation. J Cell Sci. 2018;131: pubmed publisher
Jinesh G, Kamat A. The Blebbishield Emergency Program Overrides Chromosomal Instability and Phagocytosis Checkpoints in Cancer Stem Cells. Cancer Res. 2017;77:6144-6156 pubmed publisher
Yurugi H, Marini F, Weber C, David K, Zhao Q, Binder H, et al. Targeting prohibitins with chemical ligands inhibits KRAS-mediated lung tumours. Oncogene. 2017;36:4778-4789 pubmed publisher
Taoka R, Jinesh G, Xue W, Safe S, Kamat A. CF3DODA-Me induces apoptosis, degrades Sp1, and blocks the transformation phase of the blebbishield emergency program. Apoptosis. 2017;22:719-729 pubmed publisher
Modica T, Maiorani O, Sartori G, Pivetta E, Doliana R, Capuano A, et al. The extracellular matrix protein EMILIN1 silences the RAS-ERK pathway via α4β1 integrin and decreases tumor cell growth. Oncotarget. 2017;8:27034-27046 pubmed publisher
Luhtala N, Aslanian A, Yates J, Hunter T. Secreted Glioblastoma Nanovesicles Contain Intracellular Signaling Proteins and Active Ras Incorporated in a Farnesylation-dependent Manner. J Biol Chem. 2017;292:611-628 pubmed publisher
Jinesh G, Molina J, Huang L, Laing N, Mills G, Bar Eli M, et al. Mitochondrial oligomers boost glycolysis in cancer stem cells to facilitate blebbishield-mediated transformation after apoptosis. Cell Death Discov. 2016;2:16003 pubmed publisher
Yang M, Lewinska M, Fan X, Zhu J, Yuan Z. PRR14 is a novel activator of the PI3K pathway promoting lung carcinogenesis. Oncogene. 2016;35:5527-5538 pubmed publisher
Leiser D, Medová M, Mikami K, Nisa L, Stroka D, Blaukat A, et al. KRAS and HRAS mutations confer resistance to MET targeting in preclinical models of MET-expressing tumor cells. Mol Oncol. 2015;9:1434-46 pubmed publisher
Zhang C, Li R, Jiang H, Lin S, Rogalski J, Liu K, et al. Development and application of a quantitative multiplexed small GTPase activity assay using targeted proteomics. J Proteome Res. 2015;14:967-76 pubmed publisher
Brtva T, Drugan J, Ghosh S, Terrell R, Campbell Burk S, Bell R, et al. Two distinct Raf domains mediate interaction with Ras. J Biol Chem. 1995;270:9809-12 pubmed
product information
Catalog Number :
13338
Product Name :
Raf-1 GST RBD 1-149
article :
doi10.1074/jbc.270.17.9809
id1036
pubmed_id7730360
bacterial resistance :
Ampicillin
cloning :
backbonepGEX-2T
backbone_mutation
backbone_originAmersham Pharmacia
backbone_size4950
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
pGEX-2T bacterial expression vector encoding GST followed by N-terminal residues 2-149 of human Raf-1. Contains the Ras-binding domain that binds preferentially to activated Ras-GTP. Used for determination of Ras-GTP formation in mammalian cell lysates.
origin :
37
pi :
alt_names
Raf1
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BamHI
promoter
sequencing_primer_3pGEX 3'
sequencing_primer_5pGEX 5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCMD1NN, CRAF, NS5, Raf-1, c-Raf
geneRAF1
id5894
genbank_ids
mutation
nameRaf-1
shRNA_sequence
size450
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGST
resistance markers :
114
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA