This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDR119
catalog :
13332
citations: 2
Reference
Yue J, Lu H, Lan S, Liu J, Stein M, Haffty B, et al. Identification of the DNA repair defects in a case of Dubowitz syndrome. PLoS ONE. 2013;8:e54389 pubmed publisher
Modesti M, Hesse J, Gellert M. DNA binding of Xrcc4 protein is associated with V(D)J recombination but not with stimulation of DNA ligase IV activity. EMBO J. 1999;18:2008-18 pubmed
product information
Catalog Number :
13332
Product Name :
pDR119
article :
doi10.1093/emboj/18.7.2008
id1032
pubmed_id10202163
bacterial resistance :
Kanamycin
cloning :
backbonepET28a
backbone_mutation
backbone_originNovagen
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Full length DNA ligase IV was fused to a polyhistidine tag at the C-terminus and subcloned into pET28a.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesLIG4S
geneLIG4
id3981
genbank_ids
mutation
nameDNA ligase IV
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on insert
tagHis
resistance markers :
160
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA