pcDNA3-BAP-Sox2 | Addgene 133281 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA3-BAP-Sox2

catalog :

133281

citations: 2

Reference
Kulyyassov A, Ramankulov Y, Ogryzko V. Generation of Peptides for Highly Efficient Proximity Utilizing Site-Specific Biotinylation in Cells. Life (Basel). 2022;12: pubmed publisher
Kulyyassov A, Ogryzko V. In Vivo Quantitative Estimation of DNA-Dependent Interaction of Sox2 and Oct4 Using BirA-Catalyzed Site-Specific Biotinylation. Biomolecules. 2020;10: pubmed publisher

product information

Catalog Number :

133281

Product Name :

pcDNA3-BAP-Sox2

article :

doi	10.3390/biom10010142
id	28206866
pubmed_id	31963153

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.1(+)

backbone_mutation

backbone_origin

backbone_size

6894

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

1. Kulyyassov, A. et al. PUB-MS: A Mass Spectrometry-based Method to Monitor Protein-Protein Proximity in vivo. Journal of Proteome Research10, 4416-4427, doi:10.1021/pr200189p (2011) 2. Shoaib, M. et al. PUB-NChIP- in vivo biotinylation approach to study chromatin in proximity to a protein of interest. Genome Research23, 331-340, doi:10.1101/gr.134874.111 (2013).

growth strain :

Expression of target proteins BAP-Sox2 in mammalian cells

origin :

pi :

alt_names

Sox2

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	XhoI
promoter	CMV
sequencing_primer_3	AGACGGCAATATGGTGGA
sequencing_primer_5	CTCTGGCTAACTAGAGAAC
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ANOP3, MCOPS3
gene	SOX2
id	6657

genbank_ids

NM_003106

mutation

name

Sox2

shRNA_sequence

size

956

species

9606

Homo sapiens