pIRES:mTREK-1(G137I) | Addgene 133274 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pIRES:mTREK-1(G137I)

catalog :

133274

citations: 1

Reference
Lolicato M, Arrigoni C, Mori T, Sekioka Y, Bryant C, Clark K, et al. K2P2.1 (TREK-1)-activator complexes reveal a cryptic selectivity filter binding site. Nature. 2017;547:364-368 pubmed publisher

product information

Catalog Number :

133274

Product Name :

pIRES:mTREK-1(G137I)

article :

doi	10.1038/nature22988
id	28206862
pubmed_id	28693035

bacterial resistance :

Kanamycin

cloning :

backbone

pIRES2-EGFP

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Please note: Plasmid contains the CMVd2 promoter. The plasmid works as intended, and expression levels are not affected.

growth strain :

Mammalian expression vector. It will generate the mouse TREK-1 channel full lenght G137I mutant fused to a N-terminal HA tag

origin :

pi :

alt_names

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter	CMVd2
sequencing_primer_3	CMV R
sequencing_primer_5	CMV F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	A430027H14Rik, TREK-1
gene	Kcnk2
id	16526

genbank_ids

mutation

G137I

name

Potassium channel subfamily K member 2 (KCNK2, TREK-1)

shRNA_sequence

size

species

10090

Mus musculus