This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pPICZ:mTREK-1_cryst
catalog :
133269
citations: 1
Reference
Lolicato M, Arrigoni C, Mori T, Sekioka Y, Bryant C, Clark K, et al. K2P2.1 (TREK-1)-activator complexes reveal a cryptic selectivity filter binding site. Nature. 2017;547:364-368 pubmed publisher
product information
Catalog Number :
133269
Product Name :
pPICZ:mTREK-1_cryst
article :
doi10.1038/nature22988
id28206862
pubmed_id28693035
bacterial resistance :
Bleocin (Zeocin)
cloning :
backbonepPICZ
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth strain :
P. pastoris expression vector. It will generate the mouse TREK-1 channel (21-322) fused to a C-terminal GFP
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5XhoI
promoter
sequencing_primer_3AOX3
sequencing_primer_5AOX5
site_3_destroyed1
site_5_destroyed
entrez_gene
aliasesA430027H14Rik, TREK-1
geneKcnk2
id16526
genbank_ids
mutationaa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A92P, N95S, S96D, T97Q, N119A, S300A, E306A
namePotassium channel subfamily K member 2 (KCNK2, TREK-1)
shRNA_sequence
size
species
10090
Mus musculus
tags
locationC terminal on insert
tagSNS- 3C_cleavage_site-TAAA-GFP
resistance markers :
3967
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA