This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA-DEST53 PINK1 N-GFP
catalog :
13315
citations: 3
Reference
Agarwal A, Alam M, Basu B, Pattanayak S, Asthana S, Syed G, et al. Japanese Encephalitis Virus NS4A Protein Interacts with PTEN-Induced Kinase 1 (PINK1) and Promotes Mitophagy in Infected Cells. Microbiol Spectr. 2022;10:e0083022 pubmed publisher
Siddall H, Yellon D, Ong S, Mukherjee U, Burke N, Hall A, et al. Loss of PINK1 increases the heart's vulnerability to ischemia-reperfusion injury. PLoS ONE. 2013;8:e62400 pubmed publisher
Beilina A, Van Der Brug M, Ahmad R, Kesavapany S, Miller D, Petsko G, et al. Mutations in PTEN-induced putative kinase 1 associated with recessive parkinsonism have differential effects on protein stability. Proc Natl Acad Sci U S A. 2005;102:5703-8 pubmed
product information
Catalog Number :
13315
Product Name :
pcDNA-DEST53 PINK1 N-GFP
article :
doi10.1073/pnas.0500617102
id1025
pubmed_id15824318
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA-DEST53
backbone_mutation
backbone_originInvitrogen
backbone_size7767
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please note that there is a mismatch in the linker region sequence between GFP and PINK; the amino acids WP are replaced with SG. This change does not affect any of the ORFs or features.
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3attR2
cloning_site_5attR1
promoter
sequencing_primer_3BGH-rev
sequencing_primer_5na
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBRPK, PARK6
genePINK1
id65018
genbank_ids
mutation
namePINK1
shRNA_sequence
size1743
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGFP
resistance markers :
89
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA