This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCAGGS::AtHAP2-V5
catalog :
132958
citations: 1
Reference
Valansi C, Moi D, Leikina E, Matveev E, Graña M, Chernomordik L, et al. Arabidopsis HAP2/GCS1 is a gamete fusion protein homologous to somatic and viral fusogens. J Cell Biol. 2017;216:571-581 pubmed publisher
product information
Catalog Number :
132958
Product Name :
pCAGGS::AtHAP2-V5
article :
doi10.1083/jcb.201610093
id28206813
pubmed_id28137780
bacterial resistance :
Ampicillin
cloning :
backbonepCAGGS
backbone_mutation
backbone_origin
backbone_size4741
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Express AtHAP2 full length protein in mammalian cells
origin :
37
pi :
alt_names
At GCS1
cloning
clone_methodRestriction Enzyme
cloning_site_3XmaI
cloning_site_5ClaI
promoter
sequencing_primer_3TCCCATATGTCCTTCCGAGTGA
sequencing_primer_5GCAACGTGCTGGTTATTGTGCTGTC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT1G67490, KNF, KNOPF, T1F15.4, T1F15_4, glucosidase 1
geneGCS1
id843070
aliasesAT4G11720, GCS1, GENERATIVE CELL-SPECIFIC 1, HAPLESS 2, T5C23.150, T5C23_150
geneHAP2
id826777
genbank_ids
826777
mutation
nameAtHAP2
shRNA_sequence
size2172
species
3702
Arabidopsis thaliana
tags
locationC terminal on backbone
tagV5
plasmid copy :
The A. thaliana HAP2 plasmids were derived from AtHAP2promoter::HAP2cds::YFP (PGL290; GenBank AF234315; von Besser et al., 2006) that fully rescues hap2( ) A. thaliana mutants (Wong et al., 2010).
resistance markers :
4661
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA