pGEX-4T1-14-3-3 zeta GST | Addgene 13278 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pGEX-4T1-14-3-3 zeta GST

catalog :

13278

citations: 3

Reference
Schmoker A, Weinert J, Markwood J, Albretsen K, Lunde M, Weir M, et al. FYN and ABL regulate the interaction networks of the DCBLD receptor family. Mol Cell Proteomics. 2020;: pubmed publisher
Stein B, Calzolari D, Hellberg K, Hu Y, He L, Hung C, et al. Quantitative In Vivo Proteomics of Metformin Response in Liver Reveals AMPK-Dependent and -Independent Signaling Networks. Cell Rep. 2019;29:3331-3348.e7 pubmed publisher
Yaffe M, Rittinger K, Volinia S, Caron P, Aitken A, Leffers H, et al. The structural basis for 14-3-3:phosphopeptide binding specificity. Cell. 1997;91:961-71 pubmed

product information

Catalog Number :

13278

Product Name :

pGEX-4T1-14-3-3 zeta GST

article :

doi	10.1016/S0092-8674(00)80487-0
id	1035
pubmed_id	9428519

bacterial resistance :

Ampicillin

cloning :

backbone

pGEX-4T1

backbone_mutation

backbone_origin

Amersham

backbone_size

4969

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

Insert PCR'd and cloned into pGEX-4T1 (via either EcoRI and/or BamHI?) by Tom Roberts lab. Uses stop codons of pGEX backbone rather than 14-3-3 and contains a small extra C-terminal extension. This has been corrected in the HA-tagged constructs.

origin :

pi :

alt_names

YWHAZ

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI?
cloning_site_5	EcoRI?
promoter
sequencing_primer_3
sequencing_primer_5	pGEX
site_3_destroyed
site_5_destroyed

entrez_gene

aliases
gene	YWHAZ
id	287022

genbank_ids

mutation

name

14-3-3 zeta

shRNA_sequence

size

species

9913

Bos taurus