This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGEX-2TK-14-3-3 eta GST
catalog :
13277
citations: 3
Reference
Cai N, Lou L, Al Saadi N, Tetteh S, Runnels L. The kinase activity of the channel-kinase protein TRPM7 regulates stability and localization of the TRPM7 channel in polarized epithelial cells. J Biol Chem. 2018;293:11491-11504 pubmed publisher
Feng J, Zhang Q, Li C, Zhou Y, Zhao S, Hong L, et al. Enhancement of mitochondrial biogenesis and paradoxical inhibition of lactate dehydrogenase mediated by 14-3-3? in oncocytomas. J Pathol. 2018;245:361-372 pubmed publisher
Yaffe M, Rittinger K, Volinia S, Caron P, Aitken A, Leffers H, et al. The structural basis for 14-3-3:phosphopeptide binding specificity. Cell. 1997;91:961-71 pubmed
product information
Catalog Number :
13277
Product Name :
pGEX-2TK-14-3-3 eta GST
article :
doi10.1016/S0092-8674(00)80487-0
id1035
pubmed_id9428519
bacterial resistance :
Ampicillin
cloning :
backbonepGEX-2TK
backbone_mutation
backbone_originAmersham
backbone_size4969
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Uses stop codons of pGEX-2TK rather than 14-3-3 and contains a small extra C-terminal extension. This has been corrected in the HA-tagged constructs.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BamHI
promoter
sequencing_primer_3
sequencing_primer_5pGEX
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYWHA1
geneYWHAH
id7533
genbank_ids
mutation
name14-3-3 eta
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGST
resistance markers :
595
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA