This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGEX-2TK-14-3-3 beta GST
catalog :
13276
citations: 6
Reference
Schmoker A, Weinert J, Markwood J, Albretsen K, Lunde M, Weir M, et al. FYN and ABL regulate the interaction networks of the DCBLD receptor family. Mol Cell Proteomics. 2020;: pubmed publisher
Ji H, Ostap E. The regulatory protein 14-3-3β binds to the IQ motifs of myosin-IC independent of phosphorylation. J Biol Chem. 2020;295:3749-3756 pubmed publisher
Wallroth A, Koch P, Marat A, Krause E, Haucke V. Protein kinase N controls a lysosomal lipid switch to facilitate nutrient signalling via mTORC1. Nat Cell Biol. 2019;21:1093-1101 pubmed publisher
Cai N, Lou L, Al Saadi N, Tetteh S, Runnels L. The kinase activity of the channel-kinase protein TRPM7 regulates stability and localization of the TRPM7 channel in polarized epithelial cells. J Biol Chem. 2018;293:11491-11504 pubmed publisher
Koumanov F, Richardson J, Murrow B, Holman G. AS160 phosphotyrosine-binding domain constructs inhibit insulin-stimulated GLUT4 vesicle fusion with the plasma membrane. J Biol Chem. 2011;286:16574-82 pubmed publisher
Yaffe M, Rittinger K, Volinia S, Caron P, Aitken A, Leffers H, et al. The structural basis for 14-3-3:phosphopeptide binding specificity. Cell. 1997;91:961-71 pubmed
product information
Catalog Number :
13276
Product Name :
pGEX-2TK-14-3-3 beta GST
article :
doi10.1016/S0092-8674(00)80487-0
id1035
pubmed_id9428519
bacterial resistance :
Ampicillin
cloning :
backbonepGEX-2TK
backbone_mutation
backbone_originAmersham
backbone_size4969
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Uses stop codons of pGEX-2TK rather than 14-3-3 and contains a small extra C-terminal extension. This has been corrected in the HA-tagged constructs.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BamHI
promoter
sequencing_primer_3
sequencing_primer_5pGEX
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGW128, HEL-S-1, HS1, KCIP-1, YWHAA
geneYWHAB
id7529
genbank_ids
mutation
name14-3-3 beta
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGST
resistance markers :
595
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA