This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
AAV8-hSyn-flex-miR30-eGFP-shDyn
catalog :
132716
citations: 1
Reference
Pomrenze M, Giovanetti S, Maiya R, Gordon A, Kreeger L, Messing R. Dissecting the Roles of GABA and Neuropeptides from Rat Central Amygdala CRF Neurons in Anxiety and Fear Learning. Cell Rep. 2019;29:13-21.e4 pubmed publisher
product information
Catalog Number :
132716
Product Name :
AAV8-hSyn-flex-miR30-eGFP-shDyn
article :
doi10.1016/j.celrep.2019.08.083
id28206790
pubmed_id31577943
bacterial resistance :
Ampicillin
cloning :
backbonepAAV-hSyn-flex-miR30
backbone_mutation
backbone_originAddgene Plasmid #6745
backbone_size4811
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
AAV
growth strain :
Encodes Cre-dependent short hairpin RNA (shRNA) that targets the 3 -untranslated region of the rat Pdyn gene, which encodes dynorphin
origin :
37
pi :
alt_names
GFP-shCrh fragment from pPRIME-CMV-GFP- shDyn(1) vector
cloning
clone_methodRestriction Enzyme
cloning_site_3NheI
cloning_site_5AscI
promoterSynapsin 1
sequencing_primer_3AGCAGCGTATCCACATAGCG
sequencing_primer_5TGCCTGAGAGCGCAGTCG
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
genePdyn
id29190
genbank_ids
NM_019374
mutation
nameGFP-shDyn
shRNA_sequence
size1138
species
10116
Rattus norvegicus
tags
resistance markers :
4640
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA