pPRIME-CMV-GFP-shDyn2 | Addgene 132712 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pPRIME-CMV-GFP-shDyn2

catalog :

132712

citations: 1

Reference
Pomrenze M, Giovanetti S, Maiya R, Gordon A, Kreeger L, Messing R. Dissecting the Roles of GABA and Neuropeptides from Rat Central Amygdala CRF Neurons in Anxiety and Fear Learning. Cell Rep. 2019;29:13-21.e4 pubmed publisher

product information

Catalog Number :

132712

Product Name :

pPRIME-CMV-GFP-shDyn2

article :

doi	10.1016/j.celrep.2019.08.083
id	28206790
pubmed_id	31577943

bacterial resistance :

Chloramphenicol

cloning :

backbone

pPRIME-CMV-GFP-recipient

backbone_mutation

backbone_origin

Addgene Plasmid #11657

backbone_size

8491

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Lentiviral

growth strain :

Encodes short hairpin RNA (shRNA) #2 that targets the 3 -untranslated region of the rat Pdyn gene

origin :

pi :

alt_names

small hairpin RNA targeting the 3 -untranslated regions (UTR) of rat proDynorphin

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	XhoI
promoter	CMV
sequencing_primer_3	GCTGAACGGTCTGGTTATAGG
sequencing_primer_5	TCTTGTAGTTGCCGTCGTC
site_3_destroyed
site_5_destroyed

entrez_gene

aliases
gene	Pdyn
id	29190

genbank_ids

NM_019374

mutation

name

shDyn(2)

shRNA_sequence

size

117

species

10116

Rattus norvegicus