This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
prNFL-EGFP
catalog :
132605
citations: 1
Reference
Stone E, Uchida A, Brown A. Charcot-Marie-Tooth disease Type 2E/1F mutant neurofilament proteins assemble into neurofilaments. Cytoskeleton (Hoboken). 2019;76:423-439 pubmed publisher
product information
Catalog Number :
132605
Product Name :
prNFL-EGFP
article :
doi10.1002/cm.21566
id28206752
pubmed_id31574566
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-N3
backbone_mutationPCR was used to amplify the NFL cDNA without the C-terminal myc tag using the pNFL-myc plasmid as the template. The amplified sequence was then subcloned into the multiple cloning site of the pEGFP-C1 vector, generating the following linker sequence: 5'-GGATCCATCGCCACC-3'. There is a silent mutation at nucleotide 1590G>A.
backbone_originClontech
backbone_size4698
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses EGFP-tagged rat NFL
origin :
37
pi :
alt_names
neurofilament protein L
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3SV40pA-R
sequencing_primer_5CMV Forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesNF-L, NF68, Nfl
geneNefl
id83613
genbank_ids
mutation
nameNefl
shRNA_sequence
size1656
species
10116
Rattus norvegicus
tags
locationC terminal on insert
tagEGFP
plasmid copy :
The prNFL-EGFP fluorescent fusion construct was created from a rat pNFL-myc construct provided by Chris Miller (Yates et al., Eur. J. Cell Biol. 88:193-202, 2009).
resistance markers :
1032
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA