This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
phNFM
catalog :
132597
citations: 1
Reference
Stone E, Uchida A, Brown A. Charcot-Marie-Tooth disease Type 2E/1F mutant neurofilament proteins assemble into neurofilaments. Cytoskeleton (Hoboken). 2019;76:423-439 pubmed publisher
product information
Catalog Number :
132597
Product Name :
phNFM
article :
doi10.1002/cm.21566
id28206752
pubmed_id31574566
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1 with EGFP sequence removed
backbone_mutationBefore subcloning the hNFM cDNA into the vector backbone, we added a Kozak sequence using a PCR strategy.
backbone_originClontech
backbone_size3981
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses human NFM
origin :
37
pi :
alt_names
neurofilament protein M
cloning
clone_methodRestriction Enzyme
cloning_site_3KpnI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3SV40pA-R
sequencing_primer_5CMV Forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesNEF3, NF-M, NFM
geneNEFM
id4741
genbank_ids
mutation
nameNEFM
shRNA_sequence
size3209
species
9606
Homo sapiens
tags
plasmid copy :
The human NFM cDNA was provided by Virginia Lee.
resistance markers :
1032
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA