Dynein-2_complex | Addgene 132536 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Dynein-2_complex

catalog :

132536

citations: 1

Reference
Toropova K, Zalyte R, Mukhopadhyay A, Mladenov M, Carter A, Roberts A. Structure of the dynein-2 complex and its assembly with intraflagellar transport trains. Nat Struct Mol Biol. 2019;26:823-829 pubmed publisher

product information

Catalog Number :

132536

Product Name :

Dynein-2_complex

article :

doi	10.1038/s41594-019-0286-y
id	28206735
pubmed_id	31451806

bacterial resistance :

Gentamicin

cloning :

backbone

pACEBac1

backbone_mutation

backbone_origin

Geneva Biotech

backbone_size

3000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Insect Expression

growth notes :

Cloned by Ruta Zalyte in Andrew Carter's lab. For cloning strategy, please refer to the Methods section of Toropova et al 2019.

growth strain :

Full length human (Hs) IFT dynein (dynein-2) complex for Sf9 expression. Contains His-ZZ-TEV-SNAPf-HC, WDR60, WDR34-Strep, LIC3, TCTEX-1, TCTEX1D2, LC8-1, LC8-2, TCTEX-3, Rbl-1, Rbl-2, LC8-like.

origin :

pi :

alt_names

Dynein 2 Heavy chain 1

DHC2

cloning

entrez_gene

aliases	ATD3, DHC1b, DHC2, DNCH2, DYH1B, SRPS2B, SRTD3, hdhc11
gene	DYNC2H1
id	79659

genbank_ids

mutation

Codon optimised for Sf9 expression

name

DYNC2H1

shRNA_sequence

size

12921

species

9606

Homo sapiens

tags

location	N terminal on insert
tag	8x His

location	N terminal on insert
tag	ZZ

location	N terminal on insert
tag	Linker-TEV-linker-TEV

location	N terminal on insert
tag	SNAPf

alt_names

LIC3

Cytoplasmic Dynein 2 Light Intermediate Chain 1

D2LIC

cloning

entrez_gene

aliases	CGI-60, D2LIC, LIC3
gene	DYNC2LI1
id	51626

genbank_ids

mutation

Codon optimised for Sf9 expression

name

DYNC2LI1

shRNA_sequence

size

1056

species

9606

Homo sapiens

tags

alt_names

WD repeat-containing protein 34

cloning

entrez_gene

aliases	DIC5, FAP133, SRTD11, bA216B9.3
gene	WDR34
id	89891

genbank_ids

mutation

Codon optimised for Sf9 expression

name

WDR34

shRNA_sequence

size

1695

species

9606

Homo sapiens

tags

location	C terminal on insert
tag	Strep

alt_names

WD repeat-containing protein 60

cloning

entrez_gene

aliases	FAP163, SRPS6, SRTD8
gene	WDR60
id	55112

genbank_ids

mutation

Codon optimised for Sf9 expression

name

WDR60

shRNA_sequence

size

3201

species

9606

Homo sapiens

tags

alt_names

LC8-1

Dynein Light Chain LC8-Type 1

HDLC1

cloning

entrez_gene

aliases	DLC1, DLC8, DNCL1, DNCLC1, LC8, LC8a, PIN, hdlc1
gene	DYNLL1
id	8655

genbank_ids

mutation

Codon optimised for Sf9 expression

name

DYNLL1

shRNA_sequence

size

270

species

9606

Homo sapiens

tags

alt_names

LC8-2

Dynein Light Chain LC8-Type 2

DLC2

cloning

entrez_gene

aliases	DNCL1B, Dlc2, RSPH22
gene	DYNLL2
id	140735

genbank_ids

mutation

Codon optimised for Sf9 expression

name

DYNLL2

shRNA_sequence

size

270

species

9606

Homo sapiens

tags

alt_names

Rbl-1

Roadblock-1

RB-1

cloning

entrez_gene

aliases	BITH, BLP, DNCL2A, DNLC2A, ROBLD1
gene	DYNLRB1
id	83658

genbank_ids

mutation

Codon optimised for Sf9 expression

name

DYNLRB1

shRNA_sequence

size

291

species

tags

alt_names

Roadblock-2

Rbl-2

RB-2

cloning

entrez_gene

aliases	DNCL2B, DNLC2B, ROBLD2
gene	DYNLRB2
id	83657

genbank_ids

mutation

Codon optimised for Sf9 expression

name

DYNLRB2

shRNA_sequence

size

291

species

9606

Homo sapiens

tags

alt_names

TCTEX-1

cloning

entrez_gene

aliases	CW-1, TCTEL1, tctex-1
gene	DYNLT1
id	6993

genbank_ids

mutation

Codon optimised for Sf9 expression

name

DYNLT1

shRNA_sequence

size

342

species

9606

Homo sapiens

tags

alt_names

Tctex1 domain-containing protein 2

cloning

entrez_gene

aliases	SRTD17
gene	TCTEX1D2
id	255758

genbank_ids

mutation

Codon optimised for Sf9 expression

name

TCTEX1D2

shRNA_sequence

size

429

species

9606

Homo sapiens

tags

alt_names

TCTEX-3

cloning

entrez_gene

aliases	RP3, TCTE1L, TCTEX1L
gene	DYNLT3
id	6990

genbank_ids

mutation

Codon optimised for Sf9 expression

name

DYNLT3

shRNA_sequence

size

351

species

9606

Homo sapiens

tags

alt_names

LOC392067

cloning

entrez_gene

aliases
gene	LOC392067
id	392067

genbank_ids

mutation

Codon optimised for Sf9 expression

name

LC8-like

shRNA_sequence

size

894

species

9606

Homo sapiens

tags

plasmid copy :

Cloned from genes synthesised by Epoch, codon optised for insect cell expression.

resistance markers :

2288

tags :

Unknown