AAVS1 CAG rtTA3 TauWT 2N4R-EGFP | Addgene 132389 product information

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product summary

company name :

Addgene

product type :

cDNA

product name :

AAVS1 CAG rtTA3 TauWT 2N4R-EGFP

catalog :

132389

product information

Catalog Number :

132389

Product Name :

AAVS1 CAG rtTA3 TauWT 2N4R-EGFP

article :

doi	10.21769/BioProtoc.3615
id	28206726
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

AAVS1-T2A-Puro-Cas9-TREtight

backbone_mutation

backbone_origin

Addgene

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Cre/Lox

CRISPR

growth notes :

Please note: Plasmid contains a 55 basepair deletion in the poly-GC track of the CAG promoter. It is unknown if this deletion affects plasmid function, but depositing lab was able to properly express proteins from the plasmid.

growth strain :

Exchange cassette containing inducible TauWT 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site

growth temp :

Bacteria may truncate DNA, please select multiple clones and perform restriction digest after purification to confirm.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	KpnI
cloning_site_5	BbvCI
promoter	TREtight
sequencing_primer_3
sequencing_primer_5	TTCAGGTTGGACCGGTCCTCA
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	DDPAC, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40
gene	MAPT
id	4137

genbank_ids

mutation

name

human TauWT 2N4R-EGFP

shRNA_sequence

size

species

9606

Homo sapiens

100

Synthetic