This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pJH4531
catalog :
132366
citations: 2
Reference
Banerjee N, Rojas Palato E, Shih P, Sternberg P, Hallem E. Distinct neurogenetic mechanisms establish the same chemosensory valence state at different life stages in Caenorhabditis elegans. G3 (Bethesda). 2023;: pubmed publisher
Hung W, Wang Y, Chitturi J, Zhen M. A Caenorhabditis elegans developmental decision requires insulin signaling-mediated neuron-intestine communication. Development. 2014;141:1767-79 pubmed publisher
product information
Catalog Number :
132366
Product Name :
pJH4531
article :
doi10.1242/dev.103846
id28206723
pubmed_id24671950
bacterial resistance :
Ampicillin
cloning :
backbonepBSK
backbone_mutation
backbone_originstratagene
backbone_size7430
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Worm Expression
growth notes :
This plasmid replaced plasmid pJH664 from the Zhen lab.
growth strain :
Prgef-1 DAF-2 unc-54 3' UTR C.elegans pan-neural expression of DAF-2. This plasmid has replaced plasmid pJH664 from the Zhen Lab.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3KpnI
cloning_site_5BamHI
promoterPrgef-1
sequencing_primer_3CACAATTTCATTGTTAGAGGTGAC
sequencing_primer_5gaccattaccaacgttctttc
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCELE_Y55D5A.5
genedaf-2
id175410
genbank_ids
mutation
nameDAF-2
shRNA_sequence
size6575
species
6239
Caenorhabditis elegans
tags
location
tagnone
resistance markers :
4617
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA