This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAcGFP-Hyg-CTCF-N1
catalog :
131799
citations: 1
Reference
Wang A, Han Y, Jia N, Chen P, Minden M. NPM1c impedes CTCF functions through cytoplasmic mislocalization in acute myeloid leukemia. Leukemia. 2020;34:1278-1290 pubmed publisher
product information
Catalog Number :
131799
Product Name :
pAcGFP-Hyg-CTCF-N1
article :
doi10.1038/s41375-019-0681-8
id28206706
pubmed_id31831844
bacterial resistance :
Ampicillin
cloning :
backbonepAcGFP-Hyg-N1
backbone_mutationNone
backbone_origin
backbone_size5791
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please note: Plasmid contains an IS4-like element between the AmpR promoter and AmpR resistance gene. This discrepancy is not known to affect plasmid function as plasmid was able to grow in Ampicillin.
growth strain :
Expresses human CTCF-GFP in mammalian cells through transient transfection
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5HindIII
promoter
sequencing_primer_3
sequencing_primer_5GGAG GTCTATATAA GCAGAGCTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCFAP108, FAP108, MRD21
geneCTCF
id10664
genbank_ids
mutation
nameCTCF
shRNA_sequence
size2181
species
9606
Homo sapiens
tags
locationC terminal on insert
tagGFP
plasmid copy :
Dr Elena Pugacheva
resistance markers :
4629
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA