MADR pDonor TagBFP2-V5-HRAS G12V WPRE RCE | Addgene 131730 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

MADR pDonor TagBFP2-V5-HRAS G12V WPRE RCE

catalog :

131730

citations: 1

Reference
Kim G, Rincon Fernandez Pacheco D, Saxon D, Yang A, Sabet S, Dutra Clarke M, et al. Rapid Generation of Somatic Mouse Mosaics with Locus-Specific, Stably Integrated Transgenic Elements. Cell. 2019;179:251-267.e24 pubmed publisher

product information

Catalog Number :

131730

Product Name :

MADR pDonor TagBFP2-V5-HRAS G12V WPRE RCE

article :

doi	10.1016/j.cell.2019.08.013
id	28203812
pubmed_id	31539496

bacterial resistance :

Ampicillin

cloning :

backbone

MADR pdonor

backbone_mutation

LoxP site orientation modified to be compatible with Rosa26 Cag EGFP (i.e. RCE:LoxP) mouse line from the laboratory of Gord Fishell (https://www.jax.org/strain/010701).

backbone_origin

Breunig Lab

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Cre/Lox

Synthetic Biology

Other

Mosaic analysis for dual recombinase-mediated cassette exchange (MADR) donor

growth notes :

Mutated TagBFP to TagBFP2 (I174A substitution) Compatible with RCE:loxP mouse strain (https://www.jax.org/strain/010701) but not with mt/mg strain. Can be used as a negative control for MADR insertion in mt/mg line.

growth strain :

MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusion compatible with RCE:loxP mouse strain

origin :

pi :

alt_names

cloning

clone_method	Ligation Independent Cloning
cloning_site_3
cloning_site_5
promoter	none (4x polyA to mitigate episomal expression)
sequencing_primer_3	WPRE-R (addgene)
sequencing_primer_5	MADR-MCSF (TCGACCTGCAGCCCAAGCTA)
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras
gene	HRAS
id	3265

genbank_ids

mutation

G12V

name

TagBFP2-V5-HRAS G12V

shRNA_sequence

size

species

9606

Homo sapiens

32644

Other

Entacmaea quadricolor (TagBFP)