This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLenti-puro /BRAF-V600E
catalog :
131724
citations: 1
Reference
Noeparast A, Giron P, Noor A, Bahadur Shahi R, De Brakeleer S, Eggermont C, et al. CRAF mutations in lung cancer can be oncogenic and predict sensitivity to combined type II RAF and MEK inhibition. Oncogene. 2019;38:5933-5941 pubmed publisher
product information
Catalog Number :
131724
Product Name :
pLenti-puro /BRAF-V600E
article :
doi10.1038/s41388-019-0866-7
id28206684
pubmed_id31285551
bacterial resistance :
Ampicillin
cloning :
backbonepLenti-puro (Plasmid #39481)
backbone_mutation
backbone_originIe-Ming Shih (Addgene plasmid # 39481)
backbone_size7066
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
Mammalian Expression of Flag tagged BRAF
origin :
30
pi :
alt_names
B-RAF
ENSG00000157764
cloning
clone_methodRestriction Enzyme
cloning_site_3Unknown
cloning_site_5Unknown
promoterCMV
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesB-RAF1, B-raf, BRAF-1, BRAF1, NS7, RAFB1
geneBRAF
id673
genbank_ids
mutationV600E
nameBRAF
shRNA_sequence
size2301
species
9606
Homo sapiens
tags
locationC terminal on backbone
tag3xFLAG
resistance markers :
4606
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA