This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLOC-MCOLN1
catalog :
131583
citations: 1
Reference
Kasitinon S, Eskiocak U, Martin M, Bezwada D, Khivansara V, Tasdogan A, et al. TRPML1 Promotes Protein Homeostasis in Melanoma Cells by Negatively Regulating MAPK and mTORC1 Signaling. Cell Rep. 2019;28:2293-2305.e9 pubmed publisher
product information
Catalog Number :
131583
Product Name :
pLOC-MCOLN1
article :
doi10.1016/j.celrep.2019.07.086
id28204047
pubmed_id31461647
bacterial resistance :
Ampicillin
cloning :
backbonepLOC
backbone_mutation
backbone_originDharmacon
backbone_size9089
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
For expression of human MCOLN1 in mammalian cells, GFP-tagged
origin :
37
pi :
alt_names
TRPML1, Mucolipin-1
cloning
clone_methodRestriction Enzyme
cloning_site_3NheI
cloning_site_5BamHI
promoterCMV
sequencing_primer_3
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMG-2, ML1, ML4, MLIV, MST080, MSTP080, TRP-ML1, TRPM-L1, TRPML1
geneMCOLN1
id57192
genbank_ids
mutation
nameMCOLN1
shRNA_sequence
size1743
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagturboGFP tag
plasmid copy :
The ORF was from DNASU
resistance markers :
743
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA