This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pORANGE GFP-Grin1 KI
catalog :
131485
citations: 1
Reference
Willems J, de Jong A, Scheefhals N, Mertens E, Catsburg L, Poorthuis R, et al. ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons. PLoS Biol. 2020;18:e3000665 pubmed publisher
product information
Catalog Number :
131485
Product Name :
pORANGE GFP-Grin1 KI
article :
doi10.1371/journal.pbio.3000665
id28204013
pubmed_id32275651
bacterial resistance :
Ampicillin
cloning :
backbonepORANGE
backbone_mutation
backbone_origin
backbone_size9261
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Endogenous tagging of GluN1: N-terminal (amino acid position: A20)
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3Unknown
cloning_site_5Unknown
promoterU6 and Cbh
sequencing_primer_3cgggccatttaccgtaagtt
sequencing_primer_5aaggctgttagagagataattggaa
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGluN1, NMDAR1, NR1
geneGrin1
id24408
aliasesGluN1, GluRdelta1, GluRzeta1, M100174, NMD-R1, NMDAR1, NR1, Nmdar, Rgsc174
geneGrin1
id14810
genbank_ids
mutation
namegRNA and GFP donor
shRNA_sequenceGTCTTGGGGTCGCAGGCGGCG
size
species
10090
Mus musculus
10116
Rattus norvegicus
tags
resistance markers :
4596
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA