This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET28P_dCrk
catalog :
131145
citations: 1
Reference
Spracklen A, Thornton Kolbe E, Bonner A, Florea A, Compton P, Fernandez Gonzalez R, et al. The Crk adapter protein is essential for Drosophila embryogenesis, where it regulates multiple actin-dependent morphogenic events. Mol Biol Cell. 2019;30:2399-2421 pubmed publisher
product information
Catalog Number :
131145
Product Name :
pET28P_dCrk
article :
doi10.1091/mbc.E19-05-0302
id28203978
pubmed_id31318326
bacterial resistance :
Kanamycin
cloning :
backbonepET28b
backbone_mutationthrombin cleavage site swapped out for PreScission protease cleavage site
backbone_originNovagen
backbone_size5369
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Expresses 6XHis::dCrk (based on crk isoform RA).
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5NdeI
promoter
sequencing_primer_3CCC CAA GGG GTT ATG CTA GT
sequencing_primer_5TCC CGC GAA ATT AAT ACG AC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDmel_CG1587, CG1587, CRK, D-CRK, D-Crk, DCrk, Dcrk, Dmel CG1587, crk, dCRK, dCrk
geneCrk
id43775
genbank_ids
mutation
nameCrk
shRNA_sequence
size816
species
7227
Drosophila melanogaster
tags
locationN terminal on backbone
tag6XHis
resistance markers :
4592
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA