This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pHD-dsRed-attP_dCrk_flankingHRs
catalog :
131141
citations: 1
Reference
Spracklen A, Thornton Kolbe E, Bonner A, Florea A, Compton P, Fernandez Gonzalez R, et al. The Crk adapter protein is essential for Drosophila embryogenesis, where it regulates multiple actin-dependent morphogenic events. Mol Biol Cell. 2019;30:2399-2421 pubmed publisher
product information
Catalog Number :
131141
Product Name :
pHD-dsRed-attP_dCrk_flankingHRs
article :
doi10.1091/mbc.E19-05-0302
id28203978
pubmed_id31318326
bacterial resistance :
Ampicillin
cloning :
backbonepHD-dsRed-attP
backbone_mutation
backbone_origin
backbone_size4098
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Cre/Lox
CRISPR
Other
Homology directed repair template
growth strain :
Contains ~1kb homology regions flanking Drosophila crk locus. Used to make crk null mutant. Homology regions cloned into AarI and SapI sites of pHD-dsRed-attP (Harrison, O'Connor-Giles, Wildonger).
origin :
37
pi :
alt_names
Crk
cloning
clone_methodRestriction Enzyme
cloning_site_3AarI
cloning_site_5AarI
promoter
sequencing_primer_3AAACCAGTGCAGACAATTCG
sequencing_primer_5TTCGTTTCTCGGTTTTCACC
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesDmel_CG1587, CG1587, CRK, D-CRK, D-Crk, DCrk, Dcrk, Dmel CG1587, crk, dCRK, dCrk
geneCrk
id43775
genbank_ids
mutation
nameCrk Upstream Homology Region
shRNA_sequence
size815
species
7227
Drosophila melanogaster
tags
alt_names
Crk
cloning
clone_methodRestriction Enzyme
cloning_site_3SapI
cloning_site_5SapI
promoter
sequencing_primer_3AGTGGCCGTGGCAAAAAGT
sequencing_primer_5GTGCTCTCGGAACGTGGTAT
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesDmel_CG1587, CG1587, CRK, D-CRK, D-Crk, DCrk, Dcrk, Dmel CG1587, crk, dCRK, dCrk
geneCrk
id43775
genbank_ids
mutation
nameCrk Downstream Homology Region
shRNA_sequence
size1000
species
7227
Drosophila melanogaster
tags
resistance markers :
4592
tags :
Unknown
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA