This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pTIGER_mNeonGreen::3xFLAG::dCrk
catalog :
131137
citations: 1
Reference
Spracklen A, Thornton Kolbe E, Bonner A, Florea A, Compton P, Fernandez Gonzalez R, et al. The Crk adapter protein is essential for Drosophila embryogenesis, where it regulates multiple actin-dependent morphogenic events. Mol Biol Cell. 2019;30:2399-2421 pubmed publisher
product information
Catalog Number :
131137
Product Name :
pTIGER_mNeonGreen::3xFLAG::dCrk
article :
doi10.1091/mbc.E19-05-0302
id28203978
pubmed_id31318326
bacterial resistance :
Ampicillin
cloning :
backbonepTIGER
backbone_mutation
backbone_origin
backbone_size10240
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth strain :
UASp construct for over-expression of Drosophila Crk with N-terminal monomeric NeonGreen and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3Sph1
cloning_site_5KpnI
promoter
sequencing_primer_3AACTGAGTTTCTTCGAAATTGG
sequencing_primer_5TTTGAAAACCGGTGATAGAGC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDmel_CG1587, CG1587, CRK, D-CRK, D-Crk, DCrk, Dcrk, Dmel CG1587, crk, dCRK, dCrk
geneCrk
id43775
genbank_ids
mutation
nameCrk
shRNA_sequence
size1597
species
7227
Drosophila melanogaster
tags
locationN terminal on insert
tagmNeonGreen
locationN terminal on insert
tag3XFLAG
resistance markers :
4592
tags :
Unknown
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA