This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA4/TO-ORF66 C298A-2xStrep
catalog :
131118
citations: 1
Reference
Didychuk A, Castañeda A, Kushnir L, Huang C, Glaunsinger B. Conserved CxnC motifs in Kaposi's sarcoma-associated herpesvirus ORF66 are required for viral late gene expression and are essential for its interaction with ORF34. J Virol. 2019;: pubmed publisher
product information
Catalog Number :
131118
Product Name :
pcDNA4/TO-ORF66 C298A-2xStrep
article :
doi10.1128/JVI.01299-19
id28203956
pubmed_id31578296
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA4/TO
backbone_mutation
backbone_originThermo Fisher Scientific
backbone_size5117
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses KSHV ORF66 a.a. 1-429 (full length) C298A mutant with a C-terminal 2xStrep tag in mammalian cells
origin :
37
pi :
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3TAGAAGGCACAGTCGAGG
sequencing_primer_5CGCAAATGGGCGGTAGGCGTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHHV8GK18_gp75
geneORF66
id4961523
genbank_ids
mutationORF66 a.a. 1-429 (full-length); cysteine 298 mutated to alanine
nameORF66
shRNA_sequence
size1287
species
32644
Other
Kaposi's Sarcoma Associated Herpesvirus (HHV-8)
tags
locationC terminal on backbone
tag2xStrep
resistance markers :
4579
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA